After the last microplate is placed in the reader and control is transferred to the WinKQCL™ Software to begin reading the plate, the instrument’s work is complete. At that time, it would be possible to start preparing the deck for the next run. However, the next run should not be started until all microplate readers (either 1 or 2) have completed reading the microplates, and all microplates have been removed from the readers by the analyst. When two assays are performed in a single run, the assays are performed sequentially, not concurrently. The instrument will not start the microplate setup for the second assay until the instrument has placed the microplate from the first assay in the reader to be read by the WinKQCL™ Software. When the WinKQCL™ Software begins reading the first microplate, the instrument will begin processing the microplate setup for the second assay. The system does not support an automatic retest of a failed sample. The analyst would need to add the sample to the next run to retest it.