Q:

Do we have to use ATP free tips or can we use sterile tips?

A:

When conducting the ViaLight MDA assay I have used Luria Bertani broth for my cultures. It is best if the culture media is ATP free but if it is included as the negative control on the experimental plate then this will give you an idea of the background RLU’s for the experiment. The use of sterile tips should also be fine. We routinely use tips that have been sterilised by autoclaving

Categories:
Bioassay