Q:

The animal facility which isolates my hepatocytes is at a different location, should I be concerned about transport time?

A:

Isolated hepatocytes can be transported in Krebs-Henseleit Buffer or supplemented William's E Medium. Long transport time for isolated hepatocytes, (in excess of one hour) can cause a decrease in efficiency of Nucleofection™, but does not seem to have a large effect on viability.

Categories:
Transfection