Please use always the ready-to-use protocol for the specific cell type. The optimized programs are cell type specific and do work for any kind of substrates. You can transfect small and large DNA vectors, mRNA, siRNA, gRNA, peptides and proteins with exactly the same program. This does also work for cotransfection of CRISPR complexes and Cas9 protein or Cas9 mRNA. Please check also our citation database for CRISPR publications.