Q:

Do you have a transfection protocol for cells from clouded leopard or fishing cat?

A:

Yes, we have a excellent reference for this question. The clouded leopard (Neofelis nebulosa) is currently in vulnerable status according to the International Union for Conservation of Nature red list (2017). The Zoological Park Organziation of Thailand supports many projects for reproduction and wild life conservation. Now researchers from the Burapho University in Thailand published a protocol for generation of induced pluripotent stem cells (iPSCs) from the clouded leopard. With a few somatic cells from the belly skin of the animal, episomal vectors and excisable piggyBAC transposons carrying the reprogramming factors, they used the Nucleofector technology for generation of iPSCs. After 26-30 days, the group achieved colonies with unlimited self-renewal and pluripotency similar to embryonic stem cells. Under optimal cell culture conditions, these cells can differentiate into all cell types of the body. That platform is a new tool for wildlife conservation. They also worked well for the fishing cat (Prionailurus viverrinus). I like this article because within the current immune and gene therapy publications, this is a nice new approach.

Categories:
Primary Cells and Media
Transfection
Research Areas:
Stem Cells