Q:

For generating iPSCs from PBMCs it is recommended to do a priming step to enrich the erythroblast population. Why not use T-cell or CD34+ population?

A:

It is not ideal to reprogram T-cells because of pre-existing DNA rearrangements at the V(D)J locus. While CD34+ cells work well for generating iPSCs, they are in very low numbers in the PBMC population. Erythroblasts are in relatively high numbers in PBMCs and can be expanded minimally to generate a substantial number of cells. The below reference from the Cheng lab (one of the leading labs in the generation of iPSCs) provides some details as to the reasoning to choose erythroblasts for iPSC generation from mononuclear cell populations. Chou BK, Gu H, Gao Y, Dowey SN, Wang Y, Shi J, Li Y, Ye Z, Cheng T, Cheng L. A facile method to establish human induced pluripotent stem cells from adult blood cells under feeder-free and xeno-free culture conditions: a clinically compliant approach. Stem Cells Transl Med. 2015 Apr;4(4):320-32

Related Cells:
Categories:
Primary Cells and Media
Research Areas:
Stem Cells
Basic Research