Q:

What is the difference between a Tissue Acquisition Number (TAN) and a lot/batch number?

A:

Each time Lonza receives a tissue sample, the entire tissue sample is assigned a unique Tissue Acquisition Number (TAN).
However, each collection/production of final, prepared product will be assigned a unique lot/batch number.

For many productions, Lonza will only isolate one cell type from a single piece of tissue and all product from that tissue will be processed at the same time. In such a circumstance, there would only be one TAN (for the single, initial piece of tissue) and one lot/batch number (for the single, final product).
With a different TAN per every obtained tissue, ensuring two lots/batches of a product where the donor information appears identical came from different donors becomes as simple as ensuring that the TANs are different.

However, in some cases, Lonza is able to isolate multiple cell types from a single tissue source. For example, if Lonza were to receive a piece of dermal tissue and melanocytes, keratinocytes, and fibroblasts were all isolated from the same piece of tissue, in such a circumstance, there would only be one TAN (for the single, initial piece of tissue), but there would be three, unique lot/batch numbers (for the three final produced products). With a common TAN for the three products from the same donor, finding matched sets (collections of multiple cell types isolated from the same donor) becomes as simple as finding multiple products/lots with the same TAN.

In some cases, though rarely, Lonza will only isolate one cell type from a single piece of tissue, however, the product produced from that tissue may be processed at different times. For example, if Lonza provides a product in a later passage, after initial isolation, Lonza may cryopreserve the cells in an early passage. As needed, Lonza may revive one of these early passage vials and expand it to the final product passage. In such a circumstance, there would only be one TAN (for the single, initial piece of tissue),however, each time one of the early passage vials was expanded to the final product passage would be considered a separate production and would be assigned a different lot number.

Alternatively, if a tissue sample is very large, the sample might be split with each split being processed separately. In such a circumstance, there would also only be one TAN (for the single, initial piece of tissue),however, each split of the initial tissue would be assigned a different lot number. In these cases, the majority of the TAN would be the same, however, a hyphenated number may be added to the end of the TAN. For example TAN 12345, 12345-1, and 12345-2 would all be from the same original piece of tissue and therefore from the same original donor.

Categories:
Primary Cells and Media
Research Areas:
Basic Research