Dishevelled (Dvl-2) activates canonical Wnt signalling in the absence of cytoplasmic puncta

Authors:
Smalley MJ, Signoret N, Robertson D, Tilley A, Hann A, Ewan K, Ding Y, Paterson H and Dale TC
In:
Source: J Cell Sci
Publication Date: (2005)
Issue: 118(Pt 22): 5279-5289
Research Area:
Cancer Research/Cell Biology
Platform:
Nucleofectorâ„¢ I/II/2b
Abstract
Dishevelled family proteins are multidomain intracellular transducers of Wnt signals. Ectopically expressed mammalian Dishevelled 2 (Dvl-2) activates downstream signalling and localises to cytoplasmic puncta. It has been suggested that these Dvl-2-containing structures correspond to intracellular vesicles and may be involved in the Wnt signal transduction process. We report that cytoplasmic puncta are primarily formed in cells expressing Dvl-2 at high levels. Lower levels of expression can activate signalling without forming puncta. The structures do not localise with markers of the early or late endocytic pathway and time-lapse analysis demonstrates that Dvl-2 puncta move in a random fashion over short distances but do not originate from the plasma membrane. Based on our findings, we propose that Dvl-2 puncta are protein aggregates that are not required for signalling.