Cell culture
Flp-In T-REx 293 cells (cat. no. R78007, Thermo Fisher scientific) stably expressing SA1Q were cultured and used for editing experiments as
previously described12. Ad293 cells (Cat. No. 240085, Agilent Technologies) were cultured in DMEM with 10% FBS and 1% AntibioticAntimycotic. A549 and HeLa cell lines (ACC 107 and ACC 57, DSMZ, Braunschweig, Germany) were cultured in DMEM with 10% FBS and 1% Penicillin/Streptomycin (P/S). Primary cells, NHA (Normal Human
Astrocytes, Cat.no. CC-2565) and H-RPE (Human Retinal Pigment Epithelial Cells, Cat. no. 00194987), were purchased from Lonza and
cultured in their respective commercially available medium and supplements from Lonza as instructed in the manual (AGM#- Astrocyte
Growth Medium BulletKit, Cat. no. CC-3186 for NHAs and RtEGM#- Retinal Pigment Epithelial Cell Growth Medium BulletKit Cat. no. 00195409 for H-RPE cells). HEK 293FT-cells (Cat. No. R70007, ThermoFisher Scientific) were cultured in DMEM with 10% FBS and 500 µg/ ml Geneticin. All cells were maintained at 37 °C with 5% CO2 in a watersaturated steam atmosphere. Authentication via STR profiling was performed by the commercial suppliers before purchase of the material. Cell lines were not additionally authenticated by us.