For electroporation, 20 µL of the previously generated competent C. glutamicum cells were transferred to each well of a 96-well Nucleocuvette Plate (Lonza Cologne GmbH, Cologne Germany). 3 µL of plasmid solution with a concentration of 20-50 ng µL-1 was added to each well. The electroporation plate was then placed in the 4DNucleofector 96-well Unit (Lonza Cologne GmbH, Cologne Germany) and the 4D-Nucleofector Core Unit (Lonza Cologne GmbH, Cologne Germany) was used to apply the bacterial pulse F to each well. Immediately after electroporation, 180 µL of BHISG medium prewarmed to 46 °C was added to each well. As much liquid as possible was transferred from the electroporation plate to a PCR plate and heat shocked at 46 °C for 6 min in a thermal cycler.