Engineering a 3D microfluidic culture platform for tumor-treating field application.

Pavesi A, Adriani G, Tay A, Warkiani ME, Yeap WH5, Wong SC5, Kamm RD
Source: Scientific Reports
Publication Date: (2016)
Issue: 6: 26584
Research Area:
Cancer Research/Cell Biology
Cells used in publication:
Endothelial, umbilical vein, human (HUVEC)
Species: human
Tissue Origin: vein
A 3D Microfluidic culture platform was used for treating cancer cells alone and cancer cells co-cultured with primary HUVECs using alternating electrical field therapy in a 3D extracellular matrix. The proliferation rate of the cancer cells was reduced while the endothelial cells did not show significant differences in morphology and proliferation rate.
The limitations of current cancer therapies highlight the urgent need for a more effective therapeutic strategy. One promising approach uses an alternating electric field; however, the mechanisms involved in the disruption of the cancer cell cycle as well as the potential adverse effects on non-cancerous cells must be clarified. In this study, we present a novel microfluidic device with embedded electrodes that enables the application of an alternating electric field therapy to cancer cells in a 3D extracellular matrix. To demonstrate the potential of our system to aid in designing and testing new therapeutic approaches, cancer cells and cancer cell aggregates were cultured individually or co-cultured with endothelial cells. The metastatic potential of the cancer cells was reduced after electric field treatment. Moreover, the proliferation rate of the treated cancer cells was lower compared with that of the untreated cells, whereas the morphologies and proliferative capacities of the endothelial cells were not significantly affected. These results demonstrate that our novel system can be used to rapidly screen the effect of an alternating electric field on cancer and normal cells within an in vivo-like microenvironment with the potential to optimize treatment protocols and evaluate synergies between tumor-treating field treatment and chemotherapy.