Co-electroporation with Xenopus laevis Oocytes Reprograms Normal and Cancerous Human Cells to Resemble Reprogramming Normal and Cancerous Human Cells to Resemble Induced Human Pluripotent Stem Cells

Authors:
Sergei Paylian
In:
Source: BAOJ Cancer Research & Therapy
Publication Date: (2015)
Issue: 1(2): 1
Research Area:
Cancer Research/Cell Biology
Stem Cells
Cells used in publication:
SMC, aortic (AoSMC), mouse
Species: mouse
Tissue Origin: aortic
Bone marrow stroma, human
Species: human
Tissue Origin: bone marrow
Abstract
Objective To investigate reprogramming human cells into induced pluripotent stem cells (iPSc) using co-electroporation with Xenopus laevis oocytes. Methods Human bone marrow stromal cells (BMSC), BJ cells, pre-adipocytes (HPA), CD4+ T-lymphocytes (CD4TLs), buccal mucosa cells, and HeLa and MCF-7 cells were co-electroporated with mature Xenopus laevis oocytes, cultured, and assessed for pluripotency marker expression using fluorescent immunohistochemistry. Results The co-electroporated human cells formed colonies on irradiated mouse embryonic fibroblast cells (all study cells) and StemAdhereTM substrate (assessed for co-electroporated buccal mucosa cells). Cells derived from BMSC, BJ cells, HPA, CD4TLs, and buccal mucosa cells expressed Oct 3/4, Nanog, SOX-2, Rex-1, TRA-1-60, and SSEA-1. Cells derived from co-electroporated HeLa cells expressed Oct 3/4; cells derived from co-electroporated MCF-7 cells expressed Oct 3/4 and Nanog. Reprogramming efficacy for CD4TLs was 23.4 ± 3.5%. Co-electroporated HPA trans-differentiated into neural progenitor cells in culture conditions that foster neural differentiation. Control experiments suggested that the electroporate conveyed a reprogramming factor(s). Conclusions Human cells co-electroporated with Xenopus laevis oocytes resembled iPSc in colony formation and pluripotency-associated marker expression