IPSCs were generated using non-integrating plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, and a p53 shRNA vector, introduced by nucleofection.
For more details on nucleofection please refer to the following refrence from same authors, also present in our citation database:
Transcriptome comparison of human neurons generated using induced pluripotent stem cells derived from dental pulp and skin fibroblasts. Chen J, Lin M, Foxe JJ, Pedrosa E, Hrabovsky A, Carroll R, Zheng D, Lachman HM. PLoS One. 2013 Oct 3;8(10):e75682.