Recombinant human butyrylcholinesterase as a new-age bioscavenger drug: development of the expression system

Ilyushin DG, Haertley OM, Bobik TV, Shamborant OG, Surina EA, Knorre VD, Masson P, Smirnov IV, Gabibov AG, Ponomarenko NA
Source: Acta Naturae
Publication Date: (2013)
Issue: 5(1): 73-84
Research Area:
Basic Research
Culture Media:
This paper discusses the comparison of our ProCHO 4 vs. DMEM with FBS vs. Sigma\\\'s Peprotech and Ex-Cell and the expression levels of Butyrylcholinesterase (BChE). The paper talks about the increase in production levels of the recombinant human BChE. This allows resistance to many of the never agents.
Butyrylcholinesterase (BChE) is a serine hydrolase (EC which can be found in most animal tissues. This enzyme has a broad spectrum of efficacy against organophosphorus compounds, which makes it a prime candidate for the role of stoichiometric bioscavenger. Development of a new-age DNA-encoded bioscavenger is a vival task. Several transgenic expression systems of human BChE were developed over the past 20 years; however, none of them has been shown to make economic sense or has been approved for administration to humans. In this study, a CHO-based expression system was redesigned, resulting in a significant increase in the production level of functional recombinant human butyrylcholinesterase as compared to the hitherto existing systems. The recombinant enzyme was characterized with Elman and ELISA methods.