Hematopoietic Stem Cells (HSCs) are self-renewing multipotent cells able to produce all blood cell lineages.More relevant to HSPC expansion, studies have suggested that a 3D culture microenvironment is conducive for HSPC proliferation and differentiation. The present study is an attempt to establish the appropriate ECM for long term culture of HSCs on porous bioceramic (Tricalcium phosphate, TCP) and titanium (Ti) scaffolds in order to sustain durable, long-term hematopoiesis. The Ti scaffolds (40 vol.% porosity) have been prepared through powder metallurgy route with pore size distribution in the range of 10-500µm whereas the TCP scaffolds (85 vol.% porosity) have been fabricated via direct foam casting method with layered microstructure having pore sizes in excess of 500µm. Both the scaffolds after sterilization have been used for cell culture studies. The interaction between human bone marrow derived CD34+ HSCs and the bioceramic and Ti scaffolds has been studied in proper culture conditions. The results have shown that in the presence of serum free medium, the HSCs attach to both Ti and bioceramic scaffolds and the adhesion percentage of HSCs significantly increased with the incubation time on the bioceramic scaffold compared to that on titanium scaffold.