Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola

Van Bogaert I, Zhang G, Yang J, Liu JY, Ye Y, Soetaert W, Hammock BD
Source: J Lipid Res
Publication Date: (2013)
Issue: 54(11): 3215-9
Research Area:
Basic Research
Cells used in publication:
Endothelial, umbilical vein, human (HUVEC)
Species: human
Tissue Origin: vein
The metabolism of arachidonic acid (ARA) by cytochrome P450 ?/?-1-hydroxylases leads to the formation of 20-hydroxyeicosatetraenoic acid (20-HETE), which is an important lipid-signaling molecule involved in regulation of vascular tone, angiogenesis, and inflammation. Development of a simple method to prepare 20-HETE would greatly facilitate the investigation of its biological activities. The nonpathogenic yeast Starmerella bombicola has been shown to convert exogenously added arachidonic acid to 20-HETE via the biosynthetic pathway of sophorolipids; however, the yield was low. Here we demonstrate that genetic knockout of multifunctional enzyme type 2 (MFE-2), which is involved in the ß-oxidation of fatty acids, significantly increases the yield of ARA conversion to 20-HETE and allows practical preparation of 20-HETE.