B16-F10

Skin melanoma cells

Cell Type:
Skin/Hair Epith.
Tissue Origin:
dermal
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V P-020 2e6 91% ±6 96% ±1 Plasmid (general) 2 µg 100 µl I/II/2b
SF DJ-110 2e5 50% ±1 85% ±1 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF DJ-110 1e6 67% 81% ±1 Plasmid (general) 2 µg 100 µl 4D X-Unit

Citations

Categories:
Transfection 
Authors:
Grabacka M, Plonka PM, Urbanska K, Reiss K 
In:
Clin Cancer Res (2006) 12(10): 3028-36