cells

Established from the blast cells of a 10-year-old male with biphenotypic B-myelomonocytic leukemia.

Cell Type:

Leukocyte (unspec.)

Tissue Origin:

blood

Species:

human

Research Area:

Cancer Research/Cell Biology

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Recommended Media

Iscove's Modified Dulbecco's Medium

Iscove's Modified Dulbecco's Medium (IMDM) is a modified DME containing high glucose (4,500 mg/L), sodium pyruvate, additional amino acids, HEPES buffer (in liquid form), selenium and other components. IMDM is suitable for fast growing cells and can support the growth of a wide variety of mammalian cells.

Storage = 2ºC to 8ºC

12-722 with L-glutamine
12-726 without L-glutamine
12-915 with L-glutamine and screened to support hybridoma growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
L	Q-001	2e6	29% ±4	79% ±3	Plasmid (general)	2 µg	100 µl	I/II/2b
SF	DJ-100	4e5	65% ±5	45% ±4	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SF	DJ-100	2e6	74% ±4	45% ±4	Plasmid (general)	2 µg	100 µl	4D X-Unit

Citations

DNA repair contributes to the drug-resistant phenotype of primary acute myeloid leukaemia cells with FLT3 internal tandem duplications and is reversed by the FLT3 inhibitor PKC412

Categories:

Transfection

Authors:

Seedhouse CH, Hunter HM, Lloyd-Lewis B, Massip AM, Pallis M, Carter GI, Grundy M, Shang S, Russell NH

In:

Leukemia (2006) 20(12): 2130-2136

Constitutively activated FLT3 phosphorylates BAD partially through Pim-1

Categories:

Transfection

Authors:

Kim KT, Levis M, Small D

In:

Br J of Haematol (2006) 134(5): 500-509

Block of C/EBP function by phosphorylation in acute myeloid leukemia with FLT3 activating mutations

Categories:

Transfection

Authors:

Radomska HS, Basseres DS, Zheng R, Zhang P, Dayaram T, Yamamoto Y, Sternberg DW, Lokker N, Giese NA, Bohlander SK, Schnittger S, Delmotte MH, Davis RJ, Small D, Hiddemann W, Gilliland DG and Tenen DG

In:

J Exp Med (2006) 203(2): 371-81

MV-4-11

Recommended Media

Transfection Information

Citations