IMR-32

Neuroblastoma

Cell Type:
Neuroblast
Tissue Origin:
brain
Species:
human
Research Area:
Cancer Research/Cell Biology
Neurobiology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF CA-168 2e5 85.7% ±1 62% ±3 Plasmid (general) 1 µg 20 µl 4D X-Unit
SF CA-168 1e6 83% ±1 47% ±2 Plasmid (general) 5 µg 100 µl 4D X-Unit
L C-005 1e6 80% ±2 62% ±7 Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
M Kuhlwilm, A Davierwala, S Pääbo 
In:
PLoS ONE (2013) 8 (12): e83218 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kannan A, Hettiarachchy NS, Mahedevan M 
In:
Other (2012) 3(3): 131-135 
Categories:
Transfection 
Authors:
Ohnuma-Ishikawa K, Morio T, Yamada T, Sugawara Y, Ono M, Nagasawa M, Yasuda A, Morimoto C, Ohnuma K, Dang NH, Hosoi H, Verdin E, Mizutani S 
In:
Cancer Res (2007) 67(3): 1019-29 
Categories:
Transfection 
Authors:
Subramanian C, Opipari AW Jr, Bian X, Castle VP and Kwok RP 
In:
Proc Natl Acad Sci USA (2005) 102(13): 4842-4847