NG108-15

Somatic cell hybrid of mouse neuroblastoma and rat glioma

Cell Type:
Glial Cells
Tissue Origin:
brain
Species:
cell hybrid
Research Area:
Cancer Research/Cell Biology
Neurobiology
Cell Characteristics:
Adherent

Recommended Media

Dulbecco's Modified Eagle Medium (DMEM) was developed in 1969 and is a modification of Basal Medium Eagle (BME) that differs from BME and MEM by the following characteristics:

  • Vitamins 4X greater than MEM. Vitamins and amino acids greater than BME
  • Types and quantities of amino acids greater than MEM and BME
  • Iron (ferric nitrate)

It is used in a wide range of mammalian cell culture applications.  The high glucose version is well suited to high density suspension culture. The low glucose formula is used for adherent dependent cells.

Storage = 2ºC to 8ºC

Liquid:
12-614 = 4.5 g/L glucose, without L-glutamine
12-604 = 4.5 g/L glucose, with L-glutamine
BE12-604/U1 = 4.5 g/L glucose, with Ultraglutamine I
12-914 = 4.5 g/L glucose, without L-glutamine (hybridoma screened)
12-917 = 4.5 g/L glucose, without L-glutamine or phenol red
12-709 = 4.5 g/L glucose and 25 mM HEPES, without L-glutamine
12-733= 4.5 g/L glucose, without L-glutamine or sodium pyruvate
12-741 = 4.5 g/L glucose, with L-glutamine, without sodium pyruvate

12-707 = 1.0 g/L glucose, without L-glutamine
12-708 = 1.0 g/L glucose and 25 mM HEPES, without L-glutamine

Powder:
15-614 = 4.5 g/L glucose, without L-glutamine or sodium pyruvate
15-604 = 4.5 g/L glucose, with L-glutamine or sodium pyruvate

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V X-023 1e6 64% ±2 82% ±11 Plasmid (general) 2 µg 100 µl I/II/2b
SF

CM-150

2e5 85.4% ±5 80.1% ±14 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF

CM-150

1e6 83.8% ±6 72.1% ±12 Plasmid (general) 2 µg 100 µl 4D X-Unit