WIL2-S

B lymphoblast. A HAT sensitive variant of the WIL2 human B cell line.
Cell Type:
B cell
Tissue Origin:
spleen
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V T-030 5e6 23% 49% Plasmid (general) 2 µg 100 µl I/II/2b
V T-020 5e6 22% 53% Plasmid (general) 2 µg 100 µl I/II/2b
V X-005 5e6 16% 71% Plasmid (general) 2 µg 100 µl I/II/2b