D1 ORL UVA
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Multipotent bone marrow stromal precursor cells
Cell Type:
Stromal cell
Tissue Origin:
bone marrow
Species:
mouse
Research Area:
Stem Cells
Gene Expression
Basic Research
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
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Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
T
A-033
1e6
61% ±4
97%
Plasmid (general)
2 µg
100 µl
I/II/2b
SG
EN-137
2e5
86% ±2
65% ±5
Plasmid (general)
1 µg
20 µl
4D X-Unit
SG
EN-137
1e6
87% ±1
64% ±4
Plasmid (general)
5 µg
100 µl
4D X-Unit