T84
Export
Lung metastase from colorectal carcinoma
Cell Type:
GI Tract Epith.
Tissue Origin:
colon
Species:
human
Research Area:
Cancer Research/Cell Biology
Gastroenterology
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SF
DS-138
1e6
88% ±1
51% ±6
Plasmid (general)
2 µg
100 µl
4D X-Unit
SF
DS-138
2e5
88% ±
72% ±5
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
T
T-005
2e6
52.5% ±4
83% ±8
Plasmid (general)
2 µg
100 µl
I/II/2b
SF
DS-138
2e5
88%
67-77%
Plasmid (general)
0.4 µg
20 µl
96-well
Citations
ERK activation by Neisseria gonorrhoeae downregulates epithelial cell pro-apoptotic proteins Bad and Bim
Categories:
Transfection
Authors:
Howie HL, Shiflett SL, So M
In:
Infect Immun (2008) 76(6): 2715-21
IL-22 ameliorates intestinal inflammation in a mouse model of ulcerative colitis
Categories:
Transfection
Authors:
Sugimoto K, Ogawa A, Mizoguchi E, Shimomura Y, Andoh A, Bhan AK, Blumberg RS, Xavier RJ, Mizoguchi A
In:
J Clin Invest (2008) 118(2): 534-544
Consequences of direct vs. indirect activation of EGFR in intestinal epithelial cells are dictated by protein tyrosine phosphatase 1B
Categories:
Transfection
Authors:
McCole DF, Truong A, Bunz M, Barrett KE
In:
J Biol Chem (2007) 282(18): 13303-15
HIF-1-dependent repression of equilibrative nucleoside transporter (ENT) in hypoxia
Categories:
Transfection
Authors:
Eltzschig HK, Abdulla P, Hoffman E, Hamilton KE, Daniels D, Schonfeld C, Loffler M, Reyes G, Duszenko M, Karhausen J, Robinson A, Westerman KA, Coe IR and Colgan SP
In:
J Exp Med (2005) 202(11): 1493-1505