T84

Lung metastase from colorectal carcinoma

Cell Type:
GI Tract Epith.
Tissue Origin:
colon
Species:
human
Research Area:
Cancer Research/Cell Biology
Gastroenterology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF DS-138 1e6 88% ±1 51% ±6 Plasmid (general) 2 µg 100 µl 4D X-Unit
SF DS-138 2e5 88% ± 72% ±5 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
T T-005 2e6 52.5% ±4 83% ±8 Plasmid (general) 2 µg 100 µl I/II/2b
SF DS-138 2e5 88% 67-77% Plasmid (general) 0.4 µg 20 µl Shuttle

Citations

Categories:
Transfection 
Authors:
Howie HL, Shiflett SL, So M 
In:
Infect Immun (2008) 76(6): 2715-21 
Categories:
Transfection 
Authors:
Sugimoto K, Ogawa A, Mizoguchi E, Shimomura Y, Andoh A, Bhan AK, Blumberg RS, Xavier RJ, Mizoguchi A 
In:
J Clin Invest (2008) 118(2): 534-544 
Categories:
Transfection 
Authors:
McCole DF, Truong A, Bunz M, Barrett KE 
In:
J Biol Chem (2007) 282(18): 13303-15 
Categories:
Transfection 
Authors:
Eltzschig HK, Abdulla P, Hoffman E, Hamilton KE, Daniels D, Schonfeld C, Loffler M, Reyes G, Duszenko M, Karhausen J, Robinson A, Westerman KA, Coe IR and Colgan SP 
In:
J Exp Med (2005) 202(11): 1493-1505