cells

The S2 cell line was derived in 1969 by I. Schneider from a primary culture of late stage (20-24 hours old) Drosophila melanogaster embryos.

Cell Type:

Insect

Tissue Origin:

embryo

Species:

drosophila melanogaster (fruit fly)

Cell Characteristics:

Suspension

Recommended Media

Insect-XPRESS

Insect-XPRESS™ Protein-free Insect Cell Medium is a formulation designed to support the growth of insect cell lines derived from Spodoptera frugiperda (Sf9 and Sf21).
Cell densities in excess of 8.3 x 10^6 cells/ml can be achieved with suspension cultures of Sf9 cells using Insect-XPRESS™ Medium and an excess of oxygen. This formulation can also be used for stationary mono layer cultures and shake-flask cultures.
Insect-XPRESS™ Medium contains L-glutamine and supports superior production of recombinant proteins when using the Baculovirus Expression Vector System (BEVS).

Storage = 2°C to 8°C

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
V	G-030	2e6	76% ±7	70% ±9	Plasmid (general)	2 µg	100 µl	I/II/2b
V	G-030	2e6	77% ±7	64% ±17	Plasmid (general)	2 µg	100 µl	I/II/2b
SF	DS-137	3e5	79% ±3	63% ±4	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SF	DS-137	3e5	82% ±7	84% ±11	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SF	DS-137	2e6	75% ±4	61% ±9	Plasmid (general)	2 µg	100 µl	4D X-Unit
SF	DS-137	2e6	88% ±5	78% ±5	Plasmid (general)	2 µg	100 µl	4D X-Unit

Citations (7)

Recognition determinants of broadly neutralizing human antibodies against dengue viruses.

Categories:

Serum-free and Speciality Media

Authors:

Rouvinski A, Guardado-Calvo P, Barba-Spaeth G, Duquerroy S, Vaney MC1, Kikuti CM, Navarro Sanchez ME, Dejnirattisai W, Wongwiwat W, Haouz A, Girard-Blanc C, Petres S, Shepard WE, Desprès P, Arenzana-Seisdedos F, Dussart P, Mongkolsapaya J, Screaton GR, Rey FA.

In:

Nature (2015) 520(7545): 109-13

Allosteric and hyperekplexic mutant phenotypes investigated on an a1 glycine receptor transmembrane structure.

Categories:

Serum-free and Speciality Media

Authors:

Moraga-Cid G, Sauguet L, Huon C, Malherbe L, Girard-Blanc C, Petres S, Murail S, Taly A, Baaden M, Delarue M, Corringer PJ.

In:

Proc Natl Acad Sci USA (2015) 112(9): 2865-70

Greatwall-phosphorylated Endosulfine is both an inhibitor and a substrate of PP2A-B55 heterotrimers.

Categories:

Serum-free and Speciality Media

Authors:

Williams BC1, Filter JJ, Blake-Hodek KA, Wadzinski BE, Fuda NJ, Shalloway D, Goldberg ML.

In:

eLife (2014) 3: e01695

Protein kinase Darkener of apricot and its substrate EF1gamma regulate organelle transport along microtubules.

Categories:

Serum-free and Speciality Media

Authors:

Serpinskaya AS1, Tuphile K, Rabinow L, Gelfand VI.

In:

J Cell Sci (2014) 127(Pt 1): 33-9

Molecular identification of the CRAC channel by altered ion selectivity in a mutant of Orai

Categories:

Transfection

Authors:

Yeromin AV, Zhang SL, Jiang W, Yu Y, Safrina O, Cahalan MD

In:

Nature (2006) 443(7108): 226-9

Genome-wide RNAi screen of Ca2+ influx identifies genes that regulate Ca2+ release-activated Ca2+ channel activity

Categories:

Transfection

Authors:

Zhang SL, Yeromin AV, Zhang XH, Yu Y, Safrina O, Penna A, Roos J, Stauderman KA, Cahalan MD

In:

Proc Natl Acad Sci USA (2006) 103(24): 9357-62

STIM1 is a Ca(2+) sensor that activates CRAC channels and migrates from the Ca(2+) store to the plasma membrane

Categories:

Transfection

Authors:

Zhang SL, Yu Y, Roos J, Kozak JA, Deerinck TJ, Ellisman MH, Stauderman KA and Cahalan MD

In:

Nature (2005) 437(7060): 902-905

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Schneider's Drosophila Line 2

Recommended Media

Transfection Information

Citations (7)