Schneider's Drosophila Line 2

The S2 cell line was derived in 1969 by I. Schneider from a primary culture of late stage (20-24 hours old) Drosophila melanogaster embryos.
Cell Type:
Insect
Tissue Origin:
embryo
Species:
drosophila melanogaster (fruit fly)
Cell Characteristics:
Suspension

Recommended Media

Schneider's Drosophila medium was originally developed (Schneider, I., J. Exp. Zool., 156 (1964)) for the culture of Drosophila cells (Drosophila S2 cells). It can also be used for the culture of other Dipteran cell lines.

Storage = 2ºC to 8ºC

Insect-XPRESS™ Protein-free Insect Cell Medium is a formulation designed to support the growth of insect cell lines derived from Spodoptera frugiperda (Sf9 and Sf21).
Cell densities in excess of 8.3 x 10^6 cells/ml can be achieved with suspension cultures of Sf9 cells using Insect-XPRESS™ Medium and an excess of oxygen. This formulation can also be used for stationary mono layer cultures and shake-flask cultures.
Insect-XPRESS™ Medium contains L-glutamine and supports superior production of recombinant proteins when using the Baculovirus Expression Vector System (BEVS).

Storage = 2°C to 8°C

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V G-030 2e6 76% ±7 70% ±9 Plasmid (general) 2 µg 100 µl I/II/2b
V G-030 2e6 77% ±7 64% ±17 Plasmid (general) 2 µg 100 µl I/II/2b
SF DS-137 3e5 79% ±3 63% ±4 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF DS-137 3e5 82% ±7 84% ±11 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF DS-137 2e6 75% ±4 61% ±9 Plasmid (general) 2 µg 100 µl 4D X-Unit
SF DS-137 2e6 88% ±5 78% ±5 Plasmid (general) 2 µg 100 µl 4D X-Unit

Citations

Categories:
Serum-free and Speciality Media 
Authors:
Rouvinski A, Guardado-Calvo P, Barba-Spaeth G, Duquerroy S, Vaney MC1, Kikuti CM, Navarro Sanchez ME, Dejnirattisai W, Wongwiwat W, Haouz A, Girard-Blanc C, Petres S, Shepard WE, Desprès P, Arenzana-Seisdedos F, Dussart P, Mongkolsapaya J, Screaton GR, Rey FA. 
In:
Nature (2015) 520(7545): 109-13 
Categories:
Serum-free and Speciality Media 
Authors:
Moraga-Cid G, Sauguet L, Huon C, Malherbe L, Girard-Blanc C, Petres S, Murail S, Taly A, Baaden M, Delarue M, Corringer PJ. 
In:
Proc Natl Acad Sci USA (2015) 112(9): 2865-70 
Categories:
Transfection 
Authors:
Yeromin AV, Zhang SL, Jiang W, Yu Y, Safrina O, Cahalan MD 
In:
Nature (2006) 443(7108): 226-9 
Categories:
Transfection 
Authors:
Zhang SL, Yeromin AV, Zhang XH, Yu Y, Safrina O, Penna A, Roos J, Stauderman KA, Cahalan MD 
In:
Proc Natl Acad Sci USA (2006) 103(24): 9357-62 
Categories:
Transfection 
Authors:
Zhang SL, Yu Y, Roos J, Kozak JA, Deerinck TJ, Ellisman MH, Stauderman KA and Cahalan MD 
In:
Nature (2005) 437(7060): 902-905