AGN2a

An aggressive variant of Neuro-2a derived through sequential in vivo and in vitro passaging (Johnson et al. (2003) Cell Immunol. 222:15-26)
Cell Type:
Neuroblast
Tissue Origin:
brain
Species:
mouse
Research Area:
Neurobiology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
R T-020 60-99% Plasmid (general) 1 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Johnson BD, Gershan JA, Natalia N, Zujewski H, Weber JJ, Yan X and Orentas RJ 
In:
J Immunother (2005) 28(5): 449-460 
Categories:
Transfection 
Authors:
Gershan JA, Johnson BD, Weber J, Schauer DW, Natalia N, Behnke S, Burns K, Maloney KW, Warwick AB and Orentas RJ 
In:
Genet Vaccines Ther (2005) 3(1): 4