Trypanosoma brucei

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The causative agent of human African trypanosomiasis (HAT) or African sleeping sickness. It is transmitted through the bite of the tsetse fly.

Cell Type:
Trypanosoma
Tissue Origin:
blood
Species:
unicellular
Research Area:
Parasitology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
T cell, human X-007 5e7 10% Plasmid (general) 10 µg 100 µl I/II/2b
T cell, human X-001 4e7 5.5% 60-70% Plasmid (general) 10 µg 100 µl I/II/2b
T cell, human X-001 4e7 10.8% 71.3% Plasmid (general) 10 µg 100 µl I/II/2b

Citations (9)

The Flagellar Arginine Kinase in Trypanosoma brucei Is Important for Infection in Tsetse Flies
Categories:
Transfection 
Authors:
Cher-Pheng Ooi, Brice Rotureau, Simonetta Gribaldo, Christina Georgikou, Daria Julkowska, Thierry Blisnick, Sylvie Perrot, Ines Subota, Philippe Bastin 
In:
PLoS ONE (2015) 10(7): e0133676 
Phosphatidylethanolamine and Phosphatidylcholine Biosynthesis by the Kennedy Pathway Occurs at Different Sites in Trypanosoma Brucei 
Categories:
Transfection 
Authors:
Luce Farine  1   , Moritz Niemann  3 , André Schneider  3 , Peter Bütikofer  1              
In:
Scientific Reports (2015) 18: 5 
25 years of African trypanosome research: From description to molecular dissection and new drug discovery
Categories:
Transfection 
Authors:
Matthews KR1. 
In:
Mol Biochem Parasitol (2015) 28: 0166-6851 
A novel phosphatase cascade regulates differentiation in Trypanosoma brucei via a glycosomal signaling pathway.
Categories:
Transfection 
Authors:
Szöor B, Ruberto I, Burchmore R, Matthews KR. 
In:
Genes Dev (2010) 24: 1306(16) 
Complete in vitro life cycle of Trypanosoma congolense: development of genetic tools.
Categories:
Transfection 
Authors:
Coustou V, Guegan F, Plazolles N, Baltz T. 
In:
PLoS Biol (2010) 4(3): 618 
Site-specific DNA double-strand breaks greatly increase stable transformation efficiency in Trypanosoma brucei.
Categories:
Transfection 
Authors:
Glover L, Horn D. 
In:
Mol Biochem Parasitol (2009) 166(2): 194(7) 
A Histone Methyltransferase Modulates Antigenic Variation in African Trypanosomes
Categories:
Transfection 
Authors:
Figueiredo LM, Janzen CJ, Cross GA 
In:
PLoS Biol (2008) 6(7): e161 
CRE recombinase-based positive-negative selection systems for genetic manipulation in Trypanosoma brucei
Categories:
Transfection 
Authors:
Scahill MD, Pastar I, Cross GA 
In:
Mol Biochem Parasitol (2008) 157(1): 73-82 
Highly efficient stable transformation of bloodstream forms of Trypanosoma brucei
Categories:
Transfection 
Authors:
Burkard G, Fragoso CM, Roditi I 
In:
Mol Biochem Parasitol (2007) 153(2): 220-3 
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