SU-DHL-4
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Diffuse large B-cell lymphoma. Also known as DHL4
Cell Type:
B cell
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
V
P-005
100%
siRNA
µg
100 µl
I/II/2b
R
O-017
4e6
80%
89%
Plasmid (general)
2 µg
100 µl
I/II/2b
R
O-017
5e6
100 µl
I/II/2b
SF
DS-130
6e5
CRISPR/Cas
20 µl
4D X-Unit
Citations
IRF8 regulates efficacy of therapeutic anti-CD20 monoclonal antibodies
Categories:
Transfection
Authors:
Grzelak L, Roesch F, Vaysse A, Biton A, Legendre R, Porrot F, Commère P-H, Olanchais C, Mouquet H, Vignuzzi M, Bruel T.
In:
Eur J Immunol (2022) 52: 10
Induction of autophagy and autophagy-dependent apoptosis in diffuse large B-cell lymphoma by a new antimalarial artemisinin derivative, SM1044
Categories:
Transfection
Authors:
Cheng C, Wang T, Song Z, Peng L, Gao M, Hermine O, Rousseaux S, Khochbin S, Mi JQ, Wang J.
In:
Cancer Medicine (2017) 7: 2
Cancer translocations in human cells induced by zinc finger and TALE nucleases.
Categories:
Transfection
Authors:
Piganeau M, Ghezraoui H, De Cian A, Guittat L, Tomishima M, Perrouault L, René O, Katibah GE, Zhang L, Holmes MC, Doyon Y, Concordet JP, Giovannangeli C, Jasin M, Brunet E.
In:
Genome Res (2013) 23(7): 1182-93
Cell adhesion induces p27Kip1-associated cell-cycle arrest through down-regulation of the SCFSkp2 ubiquitin ligase pathway in mantle-cell and other non-Hodgkin B-cell lymphomas
Categories:
Transfection
Authors:
Lwin T, Hazlehurst LA, Dessureault S, Lai R, Bai W, Sotomayor E, Moscinski LC, Dalton WS, Tao J
In:
Blood (2007) 110(5): 1631-8
Blockade of Hsp27 overcomes bortezomib/proteasome inhibitor PS-341 resistance in lymphoma cells
Categories:
Transfection
Authors:
Chauhan D, Li G, Shringarpure R, Podar K, Ohtake Y, Hideshima T and Anderson KC
In:
Cancer Res (2003) 63: 6174-6177
Regulation of Bcl-2 expression by C/EBP in t(14;18) lymphoma cells
Categories:
Transfection
Authors:
Heckman CA, Wheeler MA and Boxer LM
In:
Oncogene (2003) 22(39): 5947-5955