NCTC clone 929

subcutaneous connective tissue; areola and adipose subcutaneous connective tissue fibroblasts, from the normal areolar and adipose tissue of C3H/An mice. Cell Line also called L-929. Derived from the parent L strain in 1948.

Cell Type:
Adipocyte
Tissue Origin:
Connective tissue proper
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

NCTC-109 was developed in 1956 as a protein free medium for L-929 mouse cells. Its unique components include Tween® 80, nucleic acid derivations, additional vitamins, co-enzymes (DPN, CoA, TPN, FAD, UTP, TPP) and reducing agents such as glutathione, ascorbic acid and L-cystine. It is well suited for the growth of hybridoma cell lines.

Storage = 2°C to 8°C

UltraMEM Reduced Serum Medium is a chemically-defined medium designed to support growth and maintenance of several anchorage-dependent cell types under reduced serum concentrations. When supplemented with 2-4% serum, UltraMEM Medium growth performance is comparable and, in some instances, superior to that of standard media supplemented with 10% fetal bovine serum.

Growth performance in UltraMEM can be further increased by addition of insulin, transferrin, selenium, and ethanolamine [ITS or ITES] to the basal medium.

Storage = 2ºC to 8ºC 

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V X-005 1e6 67% ±2 91% ±3 Plasmid (general) 2 µg 100 µl I/II/2b
SE

FF-120

2e5 99.2% ± 65.8% ± Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SE

FF-120

1e6 99.2% ± 48.3% ±2 Plasmid (general) 2 µg 100 µl 4D X-Unit

Citations

Categories:
Transfection 
Authors:
Masih-Khan E, Trudel S, Heise C, Li Z, Paterson J, Nadeem V, Wei E, Roodman D, Claudio J, Bergsagel L, Stewart AK 
In:
Blood (2006) 108(10): 3465-71 
Categories:
Transfection 
Authors:
Yu L, Wan F, Dutta S, Welsh S, Liu Z, Freundt E, Baehrecke EH, Lenardo M 
In:
Proc Natl Acad Sci USA (2006) 103(13): 4952-7 
Categories:
Transfection 
Authors:
Thon L, Mohlig H, Mathieu S, Lange A, Bulanova E, Winoto-Morbach S, Schutze S, Bulfone-Paus S and Adam D 
In:
FASEB J (2005) 19(14): 1945-1956