NCTC clone 929

subcutaneous connective tissue; areola and adipose subcutaneous connective tissue fibroblasts, from the normal areolar and adipose tissue of C3H/An mice. Cell Line also called L-929. Derived from the parent L strain in 1948.

Cell Type:
Adipocyte
Tissue Origin:
Connective tissue proper
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE FF-120 2e5 99.2% ± 65.8% ± Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SE FF-120 1e6 99.2% ± 48.3% ±2 Plasmid (general) 2 µg 100 µl 4D X-Unit
V X-005 1e6 67% ±2 91% ±3 Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Masih-Khan E, Trudel S, Heise C, Li Z, Paterson J, Nadeem V, Wei E, Roodman D, Claudio J, Bergsagel L, Stewart AK 
In:
Blood (2006) 108(10): 3465-71 
Categories:
Transfection 
Authors:
Yu L, Wan F, Dutta S, Welsh S, Liu Z, Freundt E, Baehrecke EH, Lenardo M 
In:
Proc Natl Acad Sci USA (2006) 103(13): 4952-7 
Categories:
Transfection 
Authors:
Thon L, Mohlig H, Mathieu S, Lange A, Bulanova E, Winoto-Morbach S, Schutze S, Bulfone-Paus S and Adam D 
In:
FASEB J (2005) 19(14): 1945-1956