cells

Also called 174 x CEM.T2 - this hybrid B/T-lymphoblast cell line is a variant of the T1 cell line produced by selection with the SFR1-MI.3 monoclonal antibody (against a monomorphic determinant on HLA DR).

Cell Type:

Leukocyte (unspec.)

Tissue Origin:

blood

Species:

human

Research Area:

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SF	CA-148	4e5	60% ±10	81% ±4	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SF	CA-148	2e6	61% ±8	83% ±4	Plasmid (general)	2 µg	100 µl	4D X-Unit
C	A-030	2e6	60.4% ±8	67.8% ±7	Plasmid (general)	2 µg	100 µl	I/II/2b