Also called 174 x CEM.T2 - this hybrid B/T-lymphoblast cell line is a variant of the T1 cell line produced by selection with the SFR1-MI.3 monoclonal antibody (against a monomorphic determinant on HLA DR).

Cell Type:
Leukocyte (unspec.)
Tissue Origin:
Research Area:
Immunotherapy / Hematology
Cell Characteristics:

Recommended Media

Iscove's Modified Dulbecco's Medium (IMDM) is a modified DME containing high glucose (4,500 mg/L), sodium pyruvate, additional amino acids, HEPES buffer (in liquid form), selenium and other components. IMDM is suitable for fast growing cells and can support the growth of a wide variety of mammalian cells.

Storage = 2ºC to 8ºC

12-722 with L-glutamine
12-726 without L-glutamine
12-915 with L-glutamine and screened to support hybridoma growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
C A-030 2e6 60.4% ±8 67.8% ±7 Plasmid (general) 2 µg 100 µl I/II/2b


4e5 60% ±10 81% ±4 Plasmid (general) 0.4 µg 20 µl 4D X-Unit


2e6 61% ±8 83% ±4 Plasmid (general) 2 µg 100 µl 4D X-Unit