MDBK

Normal kidney
Cell Type:
Kidney Epith.
Tissue Origin:
kidney
Species:
bovine
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF FS-100 1e5 78% ±3 95% ±10 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF FS-100 5e5 77% ±6 81% ±10 Plasmid (general) 2 µg 100 µl 4D X-Unit
R X-001 1e6 59.3% ±6 96.2% ±5 Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Lambeth LS, Moore RJ, Muralitharan M, Dalrymple BP, McWilliam S, Doran TJ 
In:
BMC Biotechnol (2005) 5: 13