cells

Normal kidney

Cell Type:

Kidney Epith.

Tissue Origin:

kidney

Species:

bovine

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SF	FS-100	1e5	78% ±3	95% ±10	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SF	FS-100	5e5	77% ±6	81% ±10	Plasmid (general)	2 µg	100 µl	4D X-Unit
R	X-001	1e6	59.3% ±6	96.2% ±5	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations

Characterisation and application of a bovine U6 promoter for expression of short hairpin RNAs

Categories:

Transfection

Authors:

Lambeth LS, Moore RJ, Muralitharan M, Dalrymple BP, McWilliam S, Doran TJ

In:

BMC Biotechnol (2005) 5: 13

MDBK

Transfection Information

Citations