BC-1

Established from a human primary effusion lymphoma (PEL). The cells contain two viral genomes: Epstein-Barr virus (EBV) and Kaposi sarcoma associated herpesvirus (KSHV, provisionally designed HHV-8).

Cell Type:
Leukocyte (unspec.)
Tissue Origin:
Species:
human
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V

T-001

1e6 47%


Plasmid (general) 5 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Corte-Real S, Collins C, Aires da Silva F, Silmas P, Barbas Iii C, Chang Y, Moore P and Goncalves J 
In:
Blood (2005) 106(12): 3797-3802