H9 (derivative of HuT78)

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Lymphoblast - clonal derivative of the T-cell line HUT 78 derived from the blood of a patient with Sezary syndrome.

Cell Type:
T Cell
Tissue Origin:
blood
Species:
human
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V T-016 2e6 32% 48% Plasmid (general) 2 µg 100 µl I/II/2b
V G-014 2e6 57% 70% Plasmid (general) 2 µg 100 µl I/II/2b
V A-030 2e6 51% 83% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Generation of mesenchyme free intestinal organoids from human induced pluripotent stem cells
Categories:
3D Cell Culture , Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Aditya Mithal, Amalia Capilla,Dar Heinze, Andrew Berical, Carlos Villacorta-Martin, Marall Vedaie, Anjali Jacob, Kristine Abo, Aleksander Szymaniak, Megan Peasley, Alexander Stuffer, John Mahoney, Darrell N. Kotton, Finn Hawkins, and Gustavo Mostoslavsky 
In:
Nat Commun. (2020) 11: 215 
Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chal J, Al Tanoury Z, Hestin M, Gobert B, Aivio S, Hick A, Cherrier T, Nesmith AP, Parker KK, Pourquié O 
In:
Nat Protocols (2016) 11(10): 1833-50 
Efficient delivery of nuclease proteins for genome editing in human stem cells and primary cells.
Categories:
 
Authors:
Jia Liu, Thomas Gaj, Yifeng Yang, Nan Wang, Sailan Shui, Sojung Kim, Chidananda Nagamangala Kanchiswamy, Jin-Soo Kim, Carlos F Barbas III 
In:
Nat Protocols (2015) 10(11): 1842-59 
Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins
Categories:
Transfection 
Authors:
Kim S, Kim D, Cho SW, Kim J, Kim JS 
In:
Genome Res (2014) 24(6): 1012-9 
Extracellular Nef protein targets CD4+ T cells for apoptosis by interacting with CXCR4 surface receptors.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
James CO, Huang MB, Khan M, Garcia-Barrio M, Powell MD, Bond VC 
In:
J Virol (2004) 78(6): 3099-109 

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