established by G. Todaro and H. Green in 1962 from disaggregated Swiss mouse embryos.
Dulbecco's Modified Eagle Medium (DMEM) was developed in 1969 and is a modification of Basal Medium Eagle (BME) that differs from BME and MEM by the following characteristics:
It is used in a wide range of mammalian cell culture applications. The high glucose version is well suited to high density suspension culture. The low glucose formula is used for adherent dependent cells.Storage = 2ºC to 8ºCLiquid:12-614 = 4.5 g/L glucose, without L-glutamine12-604 = 4.5 g/L glucose, with L-glutamineBE12-604/U1 = 4.5 g/L glucose, with Ultraglutamine I12-914 = 4.5 g/L glucose, without L-glutamine (hybridoma screened)12-917 = 4.5 g/L glucose, without L-glutamine or phenol red12-709 = 4.5 g/L glucose and 25 mM HEPES, without L-glutamine12-733= 4.5 g/L glucose, without L-glutamine or sodium pyruvate12-741 = 4.5 g/L glucose, with L-glutamine, without sodium pyruvate12-707 = 1.0 g/L glucose, without L-glutamine12-708 = 1.0 g/L glucose and 25 mM HEPES, without L-glutaminePowder:15-614 = 4.5 g/L glucose, without L-glutamine or sodium pyruvate15-604 = 4.5 g/L glucose, with L-glutamine or sodium pyruvate
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