Fibroblast, lung, mouse
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primary fibroblasts isolated from murine lung
Cell Type:
Fibroblast
Tissue Origin:
lung
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
Fibroblast, basic
V-013
1e6
20-30%
50%
Plasmid (general)
4 µg
100 µl
I/II/2b
Citations
The aryl hydrocarbon receptor attenuates tobacco smoke induced cyclooxygenase-2 and prostaglandin production in lung fibroblasts through regulation of the NF-kappa B family member RELB
Categories:
Transfection
Authors:
Baglole CJ, Maggirwar SB, Gasiewicz TA, Thatcher TH, Phipps RP, Sime PJ
In:
J Biol Chem (2008) 283 (43): 28944-57
Fibroblast migration is mediated by CD44-dependent TGF-beta activation
Categories:
Transfection
Authors:
Acharya PS, Majumdar S, Jacob M, Hayden J, Mrass P, Weninger W, Assoian RK, Puré E
In:
J Cell Sci (2008) 121(Pt 9): 1393-402
EGF stimulates annexin 1-dependent inward vesiculation in a multivesicular endosome subpopulation
Categories:
Transfection
Authors:
White IJ, Bailey LM, Aghakhani MR, Moss SE and Futter CE
In:
EMBO J (2006) 25(1): 1-12
Single-particle tracking of murine polyoma virus-like particles on live cells and artificial membranes
Categories:
Transfection
Authors:
Ewers H, Smith AE, Sbalzarini IF, Lilie H, Koumoutsakos P and Helenius A
In:
Proc Natl Acad Sci USA (2005) 102(42): 15110-15115