1205 Lu
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Highly metastatic melanoma cell line.
Cell Type:
Skin/Hair Epith.
Tissue Origin:
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
R
K-017
1e6
90%
siRNA
1.3 µg
100 µl
I/II/2b
Citations
Identification of aurora kinase B and Wee1-like protein kinase as downstream targets of (V600E)B-RAF in melanoma.
Categories:
Transfection
Authors:
Sharma A, Madhunapantula SV, Gowda R, Berg A, Neves RI, Robertson GP.
In:
Am J Pathol (2013) 182(4): 1151-62
Targeting Mutant (V600E) B-Raf in Melanoma Interrupts Immunoediting of Leukocyte Functions and Melanoma Extravasation
Categories:
Transfection
Authors:
Liang S, Sharma A, Peng HH, Robertson G, Dong C
In:
Cancer Res (2007) 67(12): 5814-20
Targeting Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase Kinase in the Mutant (V600E) B-Raf Signaling Cascade Effectively Inhibits Melanoma Lung Metastases
Categories:
Transfection
Authors:
Sharma A, Tran MA, Liang S, Sharma AK, Amin S, Smith CD, Dong C, Robertson GP
In:
Cancer Res (2006) 66(16): 8200-9
Differential cellular responses to exogenous DNA in mammalian cells and its effect on oligonucleotide-directed gene modification
Categories:
Transfection
Authors:
Igoucheva O, Alexeev V and Yoon K
In:
Gene Ther (2006) 13(3): 266-75
Mutant V599EB-Raf regulates growth and vascular development of malignant melanoma tumors
Categories:
Transfection
Authors:
Sharma A, Trivedi NR, Zimmerman MA, Tuveson DA, Smith CD and Robertson GP
In:
Cancer Res (2005) 65(6): 412-421