Mesenchymal stem cell (MSC), rat

Bone marrow derived stem cells with the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. Generally considered a subpopulation of bone marrow stromal cells, although often the terms are used interchangeably.

Cell Type:
Blood/Bone Marrow SC
Tissue Origin:
bone marrow
Species:
rat
Research Area:
Dermatology/Tissue Engineering
Immunotherapy / Hematology
Stem Cells
Cell Characteristics:
Suspension

Recommended Media

TheraPEAK™ MSCGM™ Mesenchymal Stem Cell Growth Medium, Serum-free (with L-glutamine, without phenol red and antibiotics).

TheraPEAK™MSCGM™ is a serum free medium for the growth of human Mesenchymal Stem Cells. TheraPEAK™ MSCGM™ is optimized for the multiple passage expansion of all types of hMSCs while still allowing for differentiation into the several desired lineages. Cells can be directly transitioned from serum-containing medium to TheraPEAK™ MSCGM™ with little to no adaptation time. For peak performance, it is recommended to use TheraPEAK™ MSCGM™ with Fibronectin coated plates

UltraCULTURE™ Medium is a complete all purpose serum-free medium which supports the growth of a wide variety of both non-adherent and adherent cell lines.
The product is ready-to-use with the simple addition of 5.0 ml of L-glutamine solution (Cat. No. 17-605) per 500 ml.

The medium consists of a DMEM:F-12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The total protein concentration of UltraCULTURE™ Medium is approximately 3 mg/ml. UltraCULTURE™ Medium does not contain L-glutamine.

UltraCULTURE™ Medium may be supplemented with Cryoprotective Medium (Cat. No. 12-132) to cryopreserve cells in a serum-free environment.

Storage = 2ºC to 8ºC

Transfection Information

In case no data are shown for the selected cell type, please take a look at our optimization strategy or contact our Scientific Support Teams to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Citations

Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
de Oliveira LF, Almeida TR, Ribeiro Machado MP, Cuba MB, Alves AC, da Silva MV, Rodrigues Júnior V, Dias da Silva VJ. 
In:
Stem Cells Intl (2015) 2015: 1-12 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Onishi M, Fujita Y, Yoshikawa H, Yamashita T 
In:
Cell Death Dis. (2013) 4: e698 
Categories:
Transfection 
Authors:
Nakashima S, Matsuyama Y, Nitta A, Sakai Y and Ishiguro N 
In:
Transplant Proc (2005) 37(5): 2290-2292