293-F FreeStyle™
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suspension; Human embryonic kidney (HEK) cells transformed by adenovirus type 5
Cell Type:
Kidney Epith.
Tissue Origin:
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
V
X-001
5e5
96%
79%
Plasmid (general)
2 µg
100 µl
I/II/2b
SF
FS-100
1e6
63-79%
58-60%
Plasmid (general)
4 µg
100 µl
4D X-Unit
SF
FS-100
2e5
67-75%
58-60%
Plasmid (general)
0.8 µg
20 µl
4D X-Unit
Citations
P-selectin binds to the D'-D3 domains of von Willebrand factor in Weibel-Palade bodies
Categories:
Transfection
Authors:
Michaux G, Pullen TJ, Haberichter SL and Cutler DF
In:
Blood (2006) 107(10): 3922-4