cells

Suspension cell line derrived from the 293 cell line. The parental 293 cell line, also call HEK293, are human embryonic kidney cells transformed by adenovirus type 5

Cell Type:

Kidney Epith.

Tissue Origin:

kidney

Species:

human

Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SF	FS-100	1e6	63-79%	58-60%	Plasmid (general)	4 µg	100 µl	4D X-Unit
SF	FS-100	2e5	67-75%	58-60%	Plasmid (general)	0.8 µg	20 µl	4D X-Unit
V	X-001	5e5	96%	79%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations

P-selectin binds to the D'-D3 domains of von Willebrand factor in Weibel-Palade bodies

Categories:

Transfection

Authors:

Michaux G, Pullen TJ, Haberichter SL and Cutler DF

In:

Blood (2006) 107(10): 3922-4

293-F FreeStyle™

Transfection Information

Citations