cells

Derived from the parent HeLa cell line. The cells have been adapted to grow in suspension (spinner) culture and have the same virus susceptibility as the parent line.

Cell Type:

Uterine/Vaginal Epith.

Tissue Origin:

cervix

Species:

human

Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Suspension

Recommended Media

Basal Medium Eagle (BME)

Basal Medium Eagle is a minimal medium suitable for a variety of cell types. It is the historical precursor to Minimal Essential Medium (MEM) and was was formulated in 1955 for nutritional requirements of HeLa and mouse L cells. BME can be used for culturing primary or diploid mammalian cells.

12-105 = BME with Earle's BSS, without L-glutamine

Storage = 15ºC to 30ºC

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
L	V-001	1e6	65-69%	94-96%	Plasmid (general)	2 µg	100 µl	I/II/2b
SE	CA-123	2e5	42-48%	56-86%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SE	DS-150	2e5	78-80%	49-67%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SE	CA-123	1e6	40-52%	67-79%	Plasmid (general)	2 µg	100 µl	4D X-Unit
SE	DS-150	1e6	78-80%	56-62%	Plasmid (general)	2 µg	100 µl	4D X-Unit
SE	96-DS-150	2e5	80-90%	57-67%	Plasmid (general)	0.4 µg	20 µl	96-well
SE	96-CA-123	2e5	56-65%	93-97%	Plasmid (general)	0.4 µg	20 µl	96-well

Citations

Identification of Putative Target Genes of the Transcription Factor RUNX2

Categories:

Transfection

Authors:

M Kuhlwilm, A Davierwala, S Pääbo

In:

PLoS ONE (2013) 8 (12): e83218

HIV-1 Vpr binds to the N-lobe of Wee1 kinase domain and enhances kinase activity for Cdc2

Categories:

Transfection

Authors:

Kamata M, Watanabe N, Nagaoka Y, Chen IS

In:

J Virol (2008) 82(12): 5672-82

Quantitative analysis of Argonaute protein reveals microRNA-dependent localization to stress granules

Categories:

Transfection

Authors:

Leung AK, Calabrese JM, Sharp PA

In:

Proc Natl Acad Sci USA (2006) 103(48): 18125-30

Akt regulates the subcellular localization of the Rab27a-binding protein JFC1 by phosphorylation

Categories:

Transfection

Authors:

Johnson JL, Pacquelet S, Lane WS, Eam B and Catz SD

In:

Traffic (2005) 6(8): 667-681

Regulation of monocyte apoptosis by the protein kinase C delta (PKCdelta )-dependent phosphorylation of caspase-3

Categories:

Transfection

Authors:

Voss OH, Kim S, Wewers MD and Doseff AI

In:

J Biol Chem (2005) 280(17): 17371-17379

HeLa S3

Recommended Media

Transfection Information

Citations