Derived from the parent HeLa cell line. The cells have been adapted to grow in suspension (spinner) culture and have the same virus susceptibility as the parent line.
Basal Medium Eagle is a minimal medium suitable for a variety of cell types. It is the historical precursor to Minimal Essential Medium (MEM) and was was formulated in 1955 for nutritional requirements of HeLa and mouse L cells. BME can be used for culturing primary or diploid mammalian cells.12-105 = BME with Earle's BSS, without L-glutamine Storage = 15ºC to 30ºC
UltraCULTURE™ Medium is a complete all purpose serum-free medium which supports the growth of a wide variety of both non-adherent and adherent cell lines.The product is ready-to-use with the simple addition of 5.0 ml of L-glutamine solution (Cat. No. 17-605) per 500 ml.The medium consists of a DMEM:F-12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The total protein concentration of UltraCULTURE™ Medium is approximately 3 mg/ml. UltraCULTURE™ Medium does not contain L-glutamine.UltraCULTURE™ Medium may be supplemented with Cryoprotective Medium (Cat. No. 12-132) to cryopreserve cells in a serum-free environment.Storage = 2ºC to 8ºC
PC-1™ is a low-protein, serum-free medium intended for the culture of primary cells and anchorage-dependent cell lines. PC-1 is formulated in a specially modified DMEM/F12 base and contains a complete HEPES buffering system with known amounts of insulin, transferrin, fatty acids, and proprietary proteins assembled under strict quality control procedures. PC-1™ is intended for a variety of research and industrial applications and is formulated using defined components for optimal cell growth, while maintaining the lowest possible protein content.PC-1™ does not contain L-glutamine.PC-1™ Liquid Base Medium is to be stored at 2°C-8°C.The Supplement should be stored at -20°C.When these two components are combined, the resulting PC-1™ Complete Medium is stable for 45 days at 2°C-8°C.Once thawed, the appropriate volume of one vial of PC-1™ Supplement must be combined with the companion volume of PC-1™ Liquid Base Medium. Partial reconstitution or repeated freezing and thawing of the PC-1™ Supplement is not advised.
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