HeLa S3

Derived from the parent HeLa cell line. The cells have been adapted to grow in suspension (spinner) culture and have the same virus susceptibility as the parent line.

Cell Type:
Uterine/Vaginal Epith.
Tissue Origin:
cervix
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Suspension

Recommended Media

Basal Medium Eagle is a minimal medium suitable for a variety of cell types.  It is the historical precursor to Minimal Essential Medium (MEM) and was was formulated in 1955 for nutritional requirements of HeLa and mouse L cells.  BME can be used for culturing primary or diploid mammalian cells.

12-105 = BME with Earle's BSS, without L-glutamine 

Storage = 15ºC to 30ºC

UltraCULTURE™ Medium is a complete all purpose serum-free medium which supports the growth of a wide variety of both non-adherent and adherent cell lines.
The product is ready-to-use with the simple addition of 5.0 ml of L-glutamine solution (Cat. No. 17-605) per 500 ml.

The medium consists of a DMEM:F-12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The total protein concentration of UltraCULTURE™ Medium is approximately 3 mg/ml. UltraCULTURE™ Medium does not contain L-glutamine.

UltraCULTURE™ Medium may be supplemented with Cryoprotective Medium (Cat. No. 12-132) to cryopreserve cells in a serum-free environment.

Storage = 2ºC to 8ºC

PC-1™ is a low-protein, serum-free medium intended for the culture of primary cells and anchorage-dependent cell lines. PC-1 is formulated in a specially modified DMEM/F12 base and contains a complete HEPES buffering system with known amounts of insulin, transferrin, fatty acids, and proprietary proteins assembled under strict quality control procedures. PC-1™ is intended for a variety of research and industrial applications and is formulated using defined components for optimal cell growth, while maintaining the lowest possible protein content.
PC-1™ does not contain L-glutamine.

PC-1™ Liquid Base Medium is to be stored at 2°C-8°C.
The Supplement should be stored at -20°C.
When these two components are combined, the resulting PC-1™ Complete Medium is stable for 45 days at 2°C-8°C.

Once thawed, the appropriate volume of one vial of PC-1™ Supplement must be combined with the companion volume of PC-1™ Liquid Base Medium. Partial reconstitution or repeated freezing and thawing of the PC-1™ Supplement is not advised.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
L V-001 1e6 65-69% 94-96% Plasmid (general) 2 µg 100 µl I/II/2b
SE

CA-123

2e5 42-48% 56-86% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SE

DS-150

2e5 78-80% 49-67% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SE

CA-123

1e6 40-52% 67-79% Plasmid (general) 2 µg 100 µl 4D X-Unit
SE

DS-150

1e6 78-80% 56-62% Plasmid (general) 2 µg 100 µl 4D X-Unit
SE 96-DS-150 2e5 80-90% 57-67% Plasmid (general) 0.4 µg 20 µl Shuttle
SE

96-CA-123

2e5 56-65% 93-97% Plasmid (general) 0.4 µg 20 µl Shuttle

Citations

Categories:
Transfection 
Authors:
M Kuhlwilm, A Davierwala, S Pääbo 
In:
PLoS ONE (2013) 8 (12): e83218 
Categories:
Transfection 
Authors:
Kamata M, Watanabe N, Nagaoka Y, Chen IS 
In:
J Virol (2008) 82(12): 5672-82 
Categories:
Transfection 
Authors:
Leung AK, Calabrese JM, Sharp PA 
In:
Proc Natl Acad Sci USA (2006) 103(48): 18125-30 
Categories:
Transfection 
Authors:
Johnson JL, Pacquelet S, Lane WS, Eam B and Catz SD 
In:
Traffic (2005) 6(8): 667-681 
Categories:
Transfection 
Authors:
Voss OH, Kim S, Wewers MD and Doseff AI 
In:
J Biol Chem (2005) 280(17): 17371-17379