HeLa S3

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Derived from the parent HeLa cell line. The cells have been adapted to grow in suspension (spinner) culture and have the same virus susceptibility as the parent line.

Cell Type:
Uterine/Vaginal Epith.
Tissue Origin:
cervix
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE DS-150 2e5 80-90% 57-67% Plasmid (general) 0.4 µg 20 µl 96-well
SE CA-123 2e5 56-65% 93-97% Plasmid (general) 0.4 µg 20 µl 96-well
SE CA-123 2e5 42-48% 56-86% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SE DS-150 2e5 78-80% 49-67% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SE CA-123 1e6 40-52% 67-79% Plasmid (general) 2 µg 100 µl 4D X-Unit
SE DS-150 1e6 78-80% 56-62% Plasmid (general) 2 µg 100 µl 4D X-Unit
L V-001 1e6 65-69% 94-96% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Identification of Putative Target Genes of the Transcription Factor RUNX2
Categories:
Transfection 
Authors:
M Kuhlwilm, A Davierwala, S Pääbo 
In:
PLoS ONE (2013) 8 (12): e83218 
HIV-1 Vpr binds to the N-lobe of Wee1 kinase domain and enhances kinase activity for Cdc2
Categories:
Transfection 
Authors:
Kamata M, Watanabe N, Nagaoka Y, Chen IS 
In:
J Virol (2008) 82(12): 5672-82 
Quantitative analysis of Argonaute protein reveals microRNA-dependent localization to stress granules
Categories:
Transfection 
Authors:
Leung AK, Calabrese JM, Sharp PA 
In:
Proc Natl Acad Sci USA (2006) 103(48): 18125-30 
Akt regulates the subcellular localization of the Rab27a-binding protein JFC1 by phosphorylation
Categories:
Transfection 
Authors:
Johnson JL, Pacquelet S, Lane WS, Eam B and Catz SD 
In:
Traffic (2005) 6(8): 667-681 
Regulation of monocyte apoptosis by the protein kinase C delta (PKCdelta )-dependent phosphorylation of caspase-3
Categories:
Transfection 
Authors:
Voss OH, Kim S, Wewers MD and Doseff AI 
In:
J Biol Chem (2005) 280(17): 17371-17379 

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