SMC, bladder (BdSMC), human

Human bladder smooth muscle

Cell Type:
Smooth muscle cell
Tissue Origin:
bladder
Species:
human
Research Area:
Cancer Research/Cell Biology
Gastroenterology
Cell Characteristics:
Adherent

Recommended Media

 SmGM-2 was developed for the maintenance and expansion of human smooth muscle cells. This medium contains 5% FBS.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Smooth muscle, basic D-033 2e6 80% 90% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Franck D1, Gil ES, Adam RM, Kaplan DL, Chung YG, Estrada CR Jr, Mauney JR. 
In:
PLoS ONE (2013) 8(2): e56237 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chen CL, Cen L, Kohout J, Hutzen B, Chan C, Hsieh FC, Loy A, Huang V, Cheng G, Lin J 
In:
Mol Cancer (2008) 7: 78: 1-12