LLC-PK1

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Kidney epithelial cell line

Cell Type:
Kidney Epith.
Tissue Origin:
kidney
Species:
porcine
Research Area:
Gastroenterology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
L D-023 2e6 79% 62% Plasmid (general) 2 µg 100 µl I/II/2b
L L-029 2e6 76% 61% Plasmid (general) 2 µg 100 µl I/II/2b
L T-020 2e6 81% 66% Plasmid (general) 2 µg 100 µl I/II/2b
L T-027 2e6 78% 65% Plasmid (general) 2 µg 100 µl I/II/2b
L T-030 2e6 88% 55% Plasmid (general) 2 µg 100 µl I/II/2b
L X-005 2e6 67% 65% Plasmid (general) 2 µg 100 µl I/II/2b
L X-001 2e6 49% 81% Plasmid (general) 2 µg 100 µl I/II/2b
V X-005 2e6 58% 45% Plasmid (general) 2 µg 100 µl I/II/2b
V D-023 2e6 50% 64% Plasmid (general) 2 µg 100 µl I/II/2b
L D-023 2e6 54% 17% Plasmid (general) 2 µg 100 µl I/II/2b
SF EN-150 2e5 90% 90% Plasmid (general) 400 ng 20 µl 4D X-Unit
SF EN-138 2e5 94% 83% Plasmid (general) 400 ng 20 µl 4D X-Unit

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