MDA-MB-453
Export
mammary gland, epithelial
Cell Type:
Mammary Epith.
Tissue Origin:
breast
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SF
DK-100
4e5
75-79%
73-87%
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
SF
DK-100
2e6
87-89%
74-88%
Plasmid (general)
2 µg
100 µl
4D X-Unit
C
X-001
1e6
50-58%
84-96%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
Upregulation and activation of PKCalpha by ErbB2 through Src promotes breast cancer cell invasion that can be blocked by combined treatment with PKCalpha and Src inhibitors
Categories:
Transfection
Authors:
Tan M, Li P, Sun M, Yin G, Yu D
In:
Oncogene (2006) 25(23): 3286-95