cells

Acute myelogenous leukemia (AML). Variant subline of KG-1 cells, composed of undifferentiated promyeloblasts. Isolated by H.P. Koeffler, et al.; Blood (1980) 56: 265-273.

Cell Type:

Myeloid (unspec.)

Tissue Origin:

blood

Species:

human

Research Area:

Cancer Research/Cell Biology

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SE	DI-100	4e5	88-90%	69-71%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SE	DI-100	2e6	93-95%	70-72%	Plasmid (general)	2 µg	100 µl	4D X-Unit
L	V-001	1e6	84-88%	78-80%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations

PEP005, a selective small-molecule activator of protein kinase C, has potent antileukemic activity mediated via the delta isoform of PKC

Categories:

Transfection

Authors:

Hampson P, Chahal H, Khanim F, Hayden R, Mulder A, Assi LK, Bunce CM and Lord JM

In:

Blood (2005) 106(4): 1362-1368

KG-1a

Transfection Information

Citations