HEPA 1-6

A derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse.

Cell Type:
Liver Epith.
Tissue Origin:
liver
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Gastroenterology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF CM-138 1e5 80% 65% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF CM-137 1e5 90% 50% Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations

Categories:
Transfection 
Authors:
Yoshida Y, Hughes DE, Rausa FM 3rd, Kim IM, Tan Y, Darlington GJ, Costa RH 
In:
Hepatology (2006) 43(2): 276-86 
Categories:
Transfection 
Authors:
Hirano KI, Ikegami C, Tsujii KI, Zhang Z, Matsuura F, Nakagawa-Toyama Y, Koseki M, Masuda D, Maruyama T, Shimomura I, Ueda Y and Yamashita S 
In:
Arterioscler Thromb Vasc Biol (2005) 25(11): 2422-2427