HEPA 1-6
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A derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse.
Cell Type:
Liver Epith.
Tissue Origin:
liver
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Gastroenterology
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SF
CM-138
1e5
80%
65%
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
SF
CM-137
1e5
90%
50%
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
Citations
Chemical manipulation of an activation/inhibition switch in the nuclear receptor PXR
Categories:
Authors:
Efren Garcia-Maldonado , Andrew D Huber , Sergio C Chai , Stanley Nithianantham , Yongtao Li , Jing Wu , Shyaron Poudel , Darcie J Miller , Jayaraman Seetharaman , Taosheng Chen
In:
Nature (2024) 15: 4054
Electroporation-mediated Delivery of Cas9Ribonucleoproteins Results in High Levels of Gene Editing in Primary Hepatocytes
Categories:
Transfection
Authors:
Tanner Rathbone , Ilayda Ates , Lawrence Fernando , Ethan Addlestone , Ciaran M Lee , Vincent P Richards , Renee N Cottle de
In:
CRISPR J (2022) :
C/EBPalpha and HNF6 protein complex formation stimulates HNF6-dependent transcription by CBP coactivator recruitment in HepG2 cells
Categories:
Transfection
Authors:
Yoshida Y, Hughes DE, Rausa FM 3rd, Kim IM, Tan Y, Darlington GJ, Costa RH
In:
Hepatology (2006) 43(2): 276-86
Probucol enhances the expression of human hepatic scavenger receptor class B type I, possibly through a species-specific mechanism
Categories:
Transfection
Authors:
Hirano KI, Ikegami C, Tsujii KI, Zhang Z, Matsuura F, Nakagawa-Toyama Y, Koseki M, Masuda D, Maruyama T, Shimomura I, Ueda Y and Yamashita S
In:
Arterioscler Thromb Vasc Biol (2005) 25(11): 2422-2427