human neuroblastoma established from the supraorbital metastasis of a neuroblastoma of a 14-year-old girl in 1971.

Cell Type:
Tissue Origin:
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:

Recommended Media

MEM Eagle (also called EMEM) was developed in 1959 for cultivation of HeLa and L cells. Amino acid concentrations conform closely to the protein composition of human cells. Higher concentrations of nutrients permit longer periods between feedings. EMEM has vitamin concentrations 2-5X greater than BME and higher amino acid concentrations than BME. EMEM is suitable for culturing a broad spectrum of mammalian cells.

MEM Eagle is available with Hanks' or Earle's salts.
MEM-Hanks (12-127F or 12-137F) does not a require CO2 enriched atmosphere. 
Joklik's modification is intended for suspension culture.

Storage = 2ºC to 8ºC
                (or 15-30ºC for products 12-125, 12-136, 12-684, 12-668, 12-127 and 12-137)

MEM Eagle is available in several variations:
 - with Earle's BSS
12-611 - with L-glutamine
12-125 - without L-glutamine
12-622 - with non-essential amino acids (NEAA), and sodium pyruvate, without L-glutamine
12-136 - with L-glutamine and HEPES
12-736 - with L-glutamine, HEPES, NEAA, gentamycin, pen/strep, ampB and 2% FBS
12-684 - 10x media without sodium bicarbonate or L-glutamine
12-668 - 2x media without L-glutamine or phenol red (virus plaquing medium)
06-174 - without calcium, with NEAA and L-glutamine

- with Hank's BSS
12-127 - without L-glutamine
12-137 - with HEPES, without L-glutamine

- Joklik's Formulation

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF CM-130 2e5 80-82% 85-95% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF CM-130 1e6 80-85% 65-75% Plasmid (general) 2 µg 100 µl 4D X-Unit
V R-020 2e6 46% 50-60% Plasmid (general) 2 µg 100 µl I/II/2b
V P-016 2e6 46% 50-60% Plasmid (general) 2 µg 100 µl I/II/2b
V O-017 2e6 46% 50-60% Plasmid (general) 2 µg 100 µl I/II/2b
V S-020 2e6 80% 30% Plasmid (general) 2 µg 100 µl I/II/2b


Arocena DG, Iwahashi CK, Won N, Beilina A, Ludwig AL, Tassone F, Schwartz PH and Hagerman PJ 
Hum Mol Genet (2005) 14(23): 3661-3671