cells

human neuroblastoma established from the supraorbital metastasis of a neuroblastoma of a 14-year-old girl in 1971.

Cell Type:

Neuroblast

Tissue Origin:

brain

Species:

human

Research Area:

Cancer Research/Cell Biology

Neurobiology

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SF	CM-130	2e5	80-82%	85-95%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SF	CM-130	1e6	80-85%	65-75%	Plasmid (general)	2 µg	100 µl	4D X-Unit
V	R-020	2e6	46%	50-60%	Plasmid (general)	2 µg	100 µl	I/II/2b
V	P-016	2e6	46%	50-60%	Plasmid (general)	2 µg	100 µl	I/II/2b
V	O-017	2e6	46%	50-60%	Plasmid (general)	2 µg	100 µl	I/II/2b
V	S-020	2e6	80%	30%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations

Induction of inclusion formation and disruption of lamin A/C structure by premutation CGG-repeat RNA in human cultured neural cells

Categories:

Transfection

Authors:

Arocena DG, Iwahashi CK, Won N, Beilina A, Ludwig AL, Tassone F, Schwartz PH and Hagerman PJ

In:

Hum Mol Genet (2005) 14(23): 3661-3671

SK-N-MC

Transfection Information

Citations