SW480

adenocarcinoma
Cell Type:
GI Tract Epith.
Tissue Origin:
colon
Species:
human
Research Area:
Cancer Research/Cell Biology
Gastroenterology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V L-024 1e6 58-62% 85-87% Plasmid (general) 2 µg 100 µl I/II/2b
SF DN-100 2e5 68-76% 76-94% Plasmid (general) 0.8 µg 20 µl 4D X-Unit
SF DN-100 1e6 56-60% 65-71% Plasmid (general) 4 µg 100 µl 4D X-Unit

Citations (7)

Categories:
Transfection 
Authors:
Bianco R, Rosa R, Damiano V, Daniele G, Gelardi T, Garofalo S, Tarallo V, De Falco S, Melisi D, Benelli R, Albini A, Ryan A, Ciardiello F, Tortora G 
In:
Clin Cancer Res (2008) 14(16): 5069-80 
Categories:
Transfection 
Authors:
Niikura Y, Dixit A, Scott R, Perkins G, Kitagawa K 
In:
J Cell Biol (2007) 178(2): 283-96 
Categories:
Transfection 
Authors:
Vignjevic D, Schoumacher M, Gavert N, Janssen KP, Jih G, Laé M, Louvard D, Ben-Ze'ev A, Robine S 
In:
Cancer Res (2007) 67(14): 6844-53 
Categories:
Transfection 
Authors:
Bos CL, Diks SH, Hardwick JC, Walburg KV, Peppelenbosch MP, Richel DJ 
In:
Carcinogenesis (2006) 27(12): 2371-82 
Categories:
Transfection 
Authors:
Weiske J, Huber O 
In:
J Biol Chem (2006) 281(37): 27356-66 
Categories:
Transfection 
Authors:
Akiyoshi T, Nakamura M, Koga K, Nakashima H, Yao T, Tsuneyoshi M, Tanaka M and Katano M 
In:
Gut (2006) 55(7): 991-9 
Categories:
Transfection 
Authors:
Sakai M, Shimokawa T, Kobayashi T, Matsushima S, Yamada Y, Nakamura Y and Furukawa Y 
In:
Oncogene (2006) 25(3): 480-6 
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