HuT 78
Export
cutaneous T cell lymphoma
Cell Type:
T Cell
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SF
DF-110
2e5
48-50%
53-65%
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
SF
DF-110
1e6
45-47%
60-68%
Plasmid (general)
2 µg
100 µl
4D X-Unit
R
V-001
2e6
49-57%
53-75%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
Arrestin 3 mediates endocytosis of CCR7 following ligation of CCL19 but not CCL21
Categories:
Transfection
Authors:
Byers MA, Calloway PA, Shannon L, Cunningham HD, Smith S, Li F, Fassold BC, Vines CM
In:
J Immunol (2008) 181(7): 4723-32
CREB, ATF, and AP-1 transcription factors regulate IFN-gamma secretion by human T cells in response to mycobacterial antigen
Categories:
Transfection
Authors:
Samten B, Townsend JC, Weis SE, Bhoumik A, Klucar P, Shams H, Barnes PF
In:
J Immunol (2008) 181(3): 2056-64
Epigenetic mechanisms of age-dependent KIR2DL4 expression in T cells
Categories:
Transfection
Authors:
Li G, Weyand CM, Goronzy JJ
In:
J Leukoc Biol (2008) epub: online
The cytosolic protein talin induces an intermediate affinity integrin alphaLbeta2
Categories:
Transfection
Authors:
Li YF, Tang RH, Puan KJ, Law SK, Tan SM
In:
J Biol Chem (2007) 282(33): 24310-9
Inhibition of BCL11B expression leads to apoptosis of malignant but not normal mature T cells
Categories:
Transfection
Authors:
Grabarczyk P, Przybylski GK, Depke M, Volker U, Bahr J, Assmus K, Broker BM, Walther R, Schmidt CA
In:
Oncogene (2007) 26(26): 3797-810