HuT 78

cutaneous T cell lymphoma

Cell Type:
T Cell
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF DF-110 2e5 48-50% 53-65% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF DF-110 1e6 45-47% 60-68% Plasmid (general) 2 µg 100 µl 4D X-Unit
R V-001 2e6 49-57% 53-75% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Byers MA, Calloway PA, Shannon L, Cunningham HD, Smith S, Li F, Fassold BC, Vines CM 
In:
J Immunol (2008) 181(7): 4723-32 
Categories:
Transfection 
Authors:
Samten B, Townsend JC, Weis SE, Bhoumik A, Klucar P, Shams H, Barnes PF 
In:
J Immunol (2008) 181(3): 2056-64 
Categories:
Transfection 
Authors:
Li G, Weyand CM, Goronzy JJ 
In:
J Leukoc Biol (2008) epub: online 
Categories:
Transfection 
Authors:
Li YF, Tang RH, Puan KJ, Law SK, Tan SM 
In:
J Biol Chem (2007) 282(33): 24310-9 
Categories:
Transfection 
Authors:
Grabarczyk P, Przybylski GK, Depke M, Volker U, Bahr J, Assmus K, Broker BM, Walther R, Schmidt CA 
In:
Oncogene (2007) 26(26): 3797-810