cells

Madin-Darby Canine Cocker Spaniel kidney cell, Polarised epithelial cells, MDCK II cells were obtained from higher passage MDCK cells

Cell Type:

Kidney Epith.

Tissue Origin:

kidney

Species:

canine

Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Adherent

Recommended Media

UltraMDCK

UltraMDCK Serum-free Renal Cell Medium is designed to support the growth of MADIN-DARBY Canine Kidney (MDCK) cells at low and high plating densities.
The medium contains only two proteins: recombinant human insulin and bovine transferrin, yielding a very low protein formulation.

Storage = 2ºC to 8ºC

ProVero 1

ProVero™ 1 Serum-free Medium is a protein-free medium designed to support the growth of MDCK and vero cells. It contains HEPES and sodium bicarbonate buffer.
The absence of proteins and only very low levels of human recombinant insulin facilitate both downstream processing and regulatory compliance.

Some Vero cell strains require additional supplementation with 5.0 µg/L rhEGF for optimal vero cell growth.

Storage = 2°C to 8°C

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
L	L-005	1e6	76-84%	84-92%	Plasmid (general)	2 µg	100 µl	I/II/2b
SF	DY-100	2e5	86.9-90.9%	61.4-71.4%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SF	DY-100	1e6	91.7-93.7%	61.6-75.6%	Plasmid (general)	2 µg	100 µl	4D X-Unit
SE	CM-113	1e5	96-98%	97-100%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SE	CM-113	1e6	94%	100%	Plasmid (general)	2 µg	100 µl	4D X-Unit

Citations (8)

Contributions of galectin-3 and -9 to epithelial cell adhesion analysed by single cell force spectroscopy

Categories:

Transfection

Authors:

Friedrichs J, Torkko JM, Helenius J, Teräväinen TP, Füllekrug J, Muller DJ, Simons K, Manninen A

In:

J Biol Chem (2007) 282(40): 29375-83

AP1B sorts basolateral proteins in recycling and biosynthetic routes of MDCK cells

Categories:

Transfection

Authors:

Gravotta D, Deora A, Perret E, Oyanadel C, Soza A, Schreiner R, Gonzalez A, Rodriguez-Boulan E

In:

Proc Natl Acad Sci USA (2007) 104(5): 1564-9

Early herpes simplex virus type 1 infection is dependent on regulated Rac1/Cdc42 signalling in epithelial MDCKII cells

Categories:

Transfection

Authors:

Hoppe S, Schelhaas M, Jaeger V, Liebig T, Petermann P, Knebel-Morsdorf D

In:

J Gen Virol (2006) 87(Pt 12): 3483-94

The mammalian Scribble polarity protein regulates epithelial cell adhesion and migration through E-cadherin

Categories:

Transfection

Authors:

Qin Y, Capaldo C, Gumbiner BM and Macara IG

In:

J Cell Biol (2005) 171(6): 1061-1071

Mechanisms regulating tissue-specific polarity of monocarboxylate transporters and their chaperone CD147 in kidney and retinal epithelia

Categories:

Transfection

Authors:

Deora AA, Philp N, Hu J, Bok D and Rodriguez-Boulan E

In:

Proc Natl Acad Sci USA (2005) 102(45): 16245-16250

Gp135/podocalyxin and NHERF-2 participate in the formation of a preapical domain during polarization of MDCK cells

Categories:

Transfection

Authors:

Meder D, Shevchenko A, Simons K and Fullekrug J

In:

J Cell Biol (2005) 168(2): 303-313

Mammalian Pins is a conformational switch that links NuMA to heterotrimeric G proteins

Categories:

Transfection

Authors:

Du Q and Macara IG

In:

Cell (2004) 119(4): 503-516

Isoforms of the polarity protein par6 have distinct functions

Categories:

Transfection

Authors:

Gao L and Macara IG

In:

J Biol Chem (2004) 279(40): 41557-41562

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