MDA-MB-468

mammary gland, breast adenocarcinoma

Cell Type:
Mammary Epith.
Tissue Origin:
breast
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE DS-120 1e5 77-80% 63-81% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SE DS-120 1e6 73.6-77.2% 67.1-68.3% Plasmid (general) 2 µg 100 µl 4D X-Unit
V X-005 1e6 59-61% 77-85% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Dennler S, Andre J, Alexaki I, Li A, Magnaldo T, ten Dijke P, Wang XJ, Verrecchia F, Mauviel A 
In:
Cancer Res (2007) 67(14): 6981-6 
Categories:
Transfection 
Authors:
Park C, Lee I, Kang WK 
In:
Mol Cancer Ther (2006) 5(12): 3191-6 
Categories:
Transfection 
Authors:
Ding Y, Lee JF, Lu H, Lee MH, Yan DH 
In:
Mol Cell Biol (2006) 26(5): 1979-96 
Categories:
Transfection 
Authors:
Berglund P, Stighall M, Jirstrom K, Borgquist S, Sjolander A, Hedenfalk I and Landberg G 
In:
Cancer Res (2005) 65(21): 9727-9734 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
James CO, Huang MB, Khan M, Garcia-Barrio M, Powell MD, Bond VC 
In:
J Virol (2004) 78(6): 3099-109