U-87 MG
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glioblastoma; astrocytoma, with different genotype than U-87
Cell Type:
Glial Cells
Tissue Origin:
brain
Species:
human
Research Area:
Cancer Research/Cell Biology
Neurobiology
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
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Any Substrate
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SE
DS-126
5e5
73-83%
32-50%
Plasmid (general)
2 µg
100 µl
4D X-Unit
SE
DS-126
1e5
74-76%
39-61%
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
T
X-001
5e5
41-45%
89-93%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
Identification of Putative Target Genes of the Transcription Factor RUNX2
Categories:
Transfection
Authors:
M Kuhlwilm, A Davierwala, S Pääbo
In:
PLoS ONE (2013) 8 (12): e83218
p21 Waf1/Cip1 expression by curcumin in U-87MG human glioma cells: role of early growth response-1 expression
Categories:
Transfection
Authors:
Choi BH, Kim CG, Bae YS, Lim Y, Lee YH, Shin SY
In:
Cancer Res (2008) 68(5): 1369-77
Human immunodeficiency virus-restricted replication in astrocytes and the ability of gamma interferon to modulate this restriction are regulated by a downstream effector of the Wnt signaling pathwaysignaling pathway
Categories:
Transfection
Authors:
Carroll-Anzinger D, Kumar A, Adarichev V, Kashanchi F, Al-Harthi L
In:
J Virol (2007) 81(11): 5864-71
Phorbol 12-myristate 13-acetate induces epidermal growth factor receptor transactivation via protein kinase C delta/c-Src pathways in glioblastoma cells
Categories:
Transfection
Authors:
Amos S, Martin PM, Polar GA, Parsons SJ and Hussaini IM
In:
J Biol Chem (2005) 280(9): 7729-7738